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Large-Scale Analysis of Gene Expression Affected by HIV-1 Infection Using cDNA Microarrays A. B. VAN 'T WOUT*, S. A. MIKHEEVA, D. UPCHURCH, G. L. GEISS, M. G. KATZE, R. E. BUMGARNER, and J. I. MULLINS. Univ. of Washington, Seattle With a relatively small genome, the human immuno-deficiency virus type 1 (HIV-1) relies on host cell factors to survive and replicate. In addition, HIV-1 needs to interfere with cellular pathways aimed at destroying viral replication. Multiple cellular pathways are likely to be effected at various times during infection and by different molecular mechanisms. Ideally, one would like to examine all of the biochemical processes in a single experiment, so that the interplay between biochemical pathways can be visualized. An innovative approach that combines standard virology and molecular biology methods with recently developed robotics technology is being employed to determine the overall effect of viral infection on cellular gene expression. Using high density cDNA array technology the expression levels for 15,000 sequence verified unique human cellular cDNAs is being assessed in mock versus HIV-1 infected T cell lines at different times after infection. Customized software is then used to analyze expression patterns and identify clusters of genes belonging to specific cellular pathways affected by HIV-1. Results of several such analyses will be presented. Identification of cellular pathways affected by HIV-1 infection will likely lead to a better understanding of the pathogenesis of HIV-1 disease. Key Words: array, gene expression, hiv-1 |
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© 7th
Conference on Retroviruses and Opportunistic Infections, |