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Session 71 Poster Session IL-2 and Other Forms of Immunotherapy Session Time: 4:30-6:30 pm Room 4E-F

515-M. Long-Term Efficacy of Subcutaneous IL-2 Therapy in HIV Infection: Pro-Viral DNA in Patients of the ANRS 079 Trial M. Burgard*, C. Durier, C. Capitant, A. S. Lascaux, C. Michon, E. Netzer, C. Goujard, V. Foubert, J. P. Aboulker, J. F. Delfraissy, Y. Levy*, and C. Rouzioux for the ANRS 079 Study Group Paris, France Background: Our objective was to assess the long-term effect of IL-2 therapy on the stock of latently HIV-infected cells, by quantifying proviral DNA in PBMC. Methods: ANRS 079 is a randomized study of HAART +/- sc IL2 therapy in naïve (n=66) or pretreated patients (n=50). All patients who had frozen aliquots of PBMC were included in this sub-study of the trial ANRS 079. HIV DNA was quantified using a prototype based on the Monitor test (Roche) in 40 samples at week 0, 81 at week 28, 91 at week 72, and 64 at week 122. Results were expressed as log DNA/106 PBMC. Results: At week 72, CD4+ cell count increase was +237% in IL2 group and +76% in HAART group, HIV RNA in plasma was <50 copies/mL in 80% in IL2 group, and 87% in HAART group. After adjustment for pre-therapy, HIV DNA was 3.04 log /106 PBMC and 2.99 at week 0 in IL2 and HAART groups respectively, then 2.69 and 2.65 at week 28, 2.58, and 2.46 at week 72, 2.59, and 2.45 at week 122 (all comparisons were NS). Mean HIV DNA decrease between week 0 and 72 (n=35) was not different between IL2 group (-0.33) and HAART group (-0.45, p=0.24). Similar results were obtained for HIV DNA decreases between week 0 and week 28 (n=38) and between week 28 and week 72 (n=76). Conclusions: Our results show similar levels of decrease of HIV DNA in both groups, indicating that IL2 therapy does not induce expansion of latently HIV-infected, while CD4+ cells increase is significantly higher in patients treated with IL2.

©2002 9th Conference on Retroviruses and Opportunistic Infections