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Program

Session 37   Poster Session
Molecular Virology
4:30-6:30 pm
4E-F

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147-M. Visualizing Intracellular HIV Reverse Transcription Complexes by Immunofluorescent and Electron Microscopy
D. McDonald*1, M. Vodicka2, G. Lucero3, T. M. Svitkina4, G. G. Borisy4, M. Emerman2, and T. J. Hope1
1Univ. of Illinois, Chicago; 2Fred Hutchinson Cancer Res. Ctr., Seattle, WA; 3Salk Inst., San Diego, CA; and 4Northwestern Univ. Med. Sch., Chicago, IL
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148-M. tRNA Primer Sequences Required for Replication of Recombinant SIV Containing HIV-1 Reverse Transcriptase
L. Alexander* and K. Soderberg
Yale Univ., New Haven, CT
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149-M.
Effects of 69S-XX Finger Insertion Mutations in HIV-1 Reverse Transcriptase on Nucleotide-Dependent Removal of ddA Monophosphate from Blocked DNA Chains
P. Meyer*1, J. Lennerstrand2, S. Matsuura1, B. Larder3, and W. Scott1
1 Univ. of Miami, FL; 2Huddinge Univ. Hosp., Stockholm, Sweden; and 3 Virco, Cambridge, UK
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150-M. Mutations in the p51-RNase H Protease Cleavage Junction Effects Intravirion Stability of HIV-1 Reverse Transcriptase
M. Abram*, D. Arion, N. Sluis-Cremer, and M. Parniak
Univ. of Pittsburgh Sch. of Med., PA
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151-M. The Tat Protein of HIV-1 can Promote the Annealing of tRNALys3 onto the Primer-Binding Site (PBS) and can also Promote the First Strand Transfer in Reverse Transcription
M. A. Wainberg*, C. Liang, X. Guo, and M. Kameoka
McGill Univ. AIDS Ctr., Montreal, QC, Canada
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152-M.
Regulation of the HIV-1 Pre-Integration Complex (PIC) in Primary Human Lymphocytes
A. Brooun1, D. Chinnasamy2, V. Snarsky1, C. Spina1, D. D. Richman1, F. Candotti2, and R. Kornbluth*1
1Univ. of California, San Diego and VA San Diego Healthcare System, La Jolla, CA and 2Human Genome Res. Inst., NIH, Bethesda, MD
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153-M. Late HIV-1 Reverse Transcripts Accumulate Stably within Resting CD4(+) T Cells
W. Swiggard*1, U. O'Doherty1, D. McGain1, D. Jeyakumar1, and M. Malim2
1Univ. of Pennsylvania, Philadelphia and 2King's Coll., London, UK
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154-M. Differential Interaction with Actin by HIV-1 Gag and Matrix Protein
C. Gomez* and T. Hope
Univ. of Illinois, Chicago
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155-M. Investigating Retroviral Trafficking
M. Vodicka*1, M. Imakura1, D. McDonald2, T. J. Hope2, and M. Emerman1
1Fred Hutchinson Cancer Res. Ctr., Seattle, WA and 2Univ. of Illinois, Chicago
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156-M. A Sensitive, Quantitative Assay for HIV-1 Integration
U. O'Doherty*1, W. Swiggard1, D. Jeyakumar1, D. McGain1, and M. Malim2
1Univ. of Pennsylvania, Philadelphia and 2King's Coll., London, UK
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157-M. Analysis of 2-LTR Circle Stability and Gene Expression in Primary Human Macrophages
T. Cunningham*, J. Mediavilla, M. Pope, and M. Muesing
Aaron Diamond AIDS Res. Ctr., Rockefeller Univ., and Population Council, Ctr. for BioMed. Res., New York, NY
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158-M. HIV-1 Extrachromosomal Circular DNA is Long-Lived in Non-Dividing Cells and Proteins Expressed from the Circles May Play a Role in Viral Pathogenesis
L. Gillim*, A. Cara, and M. E. Klotman
Mount Sinai Sch. of Med., New York, NY
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159-M. Longitudinal Studies of 2-LTR Circles in Patients on CART
J. Morlese*, I. Teo, J. W. Choi, B. Gazzard, and S. Shaunak
Imperial Coll. at Hammersmith and Chelsea & Westminster Hosp., London, UK
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160-M. Characterization of the Pre-Integration State of HIV-1 Latency
T. Pierson*1, 2, Y. Zhou1, T. Kieffer1, C. Ruff1, and R. Siliciano1
1Johns Hopkins Sch. of Med., Baltimore, MD and 2Univ. of Pennsylvania, Philadelphia
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161-M.
Exposing the Latent Reservoir of Human Immunodeficiency Virus Type-1 (HIV-1) via Blockade of Host Repressors
D. M. Margolis*1,3, G. He1, L. Ylisastigui1, V. C. Rucker2, and P. B. Dervan2
1Univ. of Texas Southwestern Med. Ctr., Dallas; 2California Inst. of Technology, Pasadena; and 3North Texas Veterans Hlth. Care Systems, Dallas
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162-M. A Dual Role for Leader Sequences Downstream of the SIV Stem-Loop 1 in RNA Packaging and Dimerization of Simian Immunodeficiency Virus RNA
J. B. Whitney*1, 2, M. Olivera1, B. Spira1, Y. Guan1, M. A. Wainberg1, and 2
1McGill AIDS Ctr., Lady Davis Inst. and 2McGill Univ., Montreal, QC, Canada
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163-M. Identification of Novel Sequences Involved in HIV-1 Genomic RNA Dimerization
R. S. Russell*, J. Hu, M. A. Wainberg, and C. Liang
McGill AIDS Ctr., Lady Davis Inst., Jewish Gen. Hosp., Montreal, QC, Canada
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164-M.
Transduction of Cellular Sequence by Human Immunodeficiency Virus Type 1
G. Sun*1, P-K. O’Neil2, Y. Ron1, B-D. Preston2, and J. P. Dougherty1
1Univ. of Med., and Dentistry, New Jersey-Robert Wood Johnson Med. Sch., Piscataway and 2Univ. of Utah, Salt Lake City
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165-M. HIV-1 Protease Dimerization and Virus Assembly
S. Pettit, L. Everitt, S. Choudhury, and A. H. Kaplan*
Univ. of North Carolina, Chapel Hill
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166-M. Temporal Modulation of Monocyte-Derived Macrophage Gene Expression During HIV-1 Infection
F. Ottones, C. Royer, and J. Corbeil*
Univ. of California, San Diego and Veterans Med. Res. Fndn., La Jolla, CA
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167-M.
HIV-1 Nef-Mediated Recruitment of Adaptor Protein Complexes
D. Hitchin1, K. Janvier2, H. Craig1, S. Coleman*1, S. Benichou2, and J. Guatelli1
1Univ. of California, San Diego Med. Ctr. and 2INSERM, Inst. Cochin de Genetique Moleculaire, Paris, France
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168-M.
Gene Expression Studies of in Vitro HIV-1 Infection: HIV-1 Activates Transcription of the Sterol Biosynthesis Pathway
A. van't Wout*, G. Lehrman, S. Mikheeva, G. O’Keeffe, G. Geiss, R. Bumgarner, and J. Mullins
Univ. of Washington, Seattle
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169-M.
Identification of a Recombination Hot Spot in HIV-1 Gag
C. Dykes*, V. Planelles, Y. Zhu, and L. M. Demeter
Univ. of Rochester Sch. of Med. and Dentistry, NY
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170-M.
Differences in Genetic Recombination during HIV-1 and MLV Replication
A. Onafuwa*, W. An, N. Robson, and A. Telesnitsky
Univ. of Michigan Med. Sch., Ann Arbor
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171-M. A Role for PI3-Kinase Signaling during HIV Infection of Primary CD4+ T Lymphocytes and Macrophages
F. Francois*,, and M. E. Klotman
Mt. Sinai Sch. of Med., New York, NY
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172-M.
Apoptosis Signal to CD4+ T Cells in HIV-1 Infected Hu-PBL-SCID Mice
Y. Koyanagi* and Y. Miura
Tohoku Univ. Graduate Sch. of Med., Sendai, Japan and Tokyo Med. and Dental Univ., Japan
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173-M.
Human T-Cell Leukemia Virus Type-I Buds from Lipid Rafts
K. Niyogi* and J. Hildreth
Johns Hopkins Univ. Sch. of Med., Baltimore, MD
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