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Session 53   Poster Abstracts
Viral Envelopes: Structure-Function Studies
Monday, 1:30 - 3:30 pm
Poster Hall
290HIV-1 Acute Infection Env Glycomutants Designed from 3D-Model: Effects on Processing, Antigenicity and Neutralization Sensitivity
F Reynard*, B Verrier, and F Bedin
UMR2142 CNRS/bioMérieux, Lyon, France
291Molecular Characterization of env genes From Primary HIV-2 Strains Unable to Use CCR5 or CXCR4 Co-receptors
Q Santos-Costa*1, R G Collman2, J Moniz-Pereira1, and J M Azevedo-Pereira1
1Univ. of Lisbon, Faculty of Pharmacy, Ctr. of Molecular Pathogenesis, Portugal and 2Univ. of Pennsylvania Sch. of Med., Philadelphia, USA
292Functional Analysis of the HIV-1 Envelope Bridging Sheet
M Biscone*, J Reeves, F Baribaud, S Baik, and R Doms
Univ. of Pennsylvania, Philadelphia, USA
293Characterization of HIV-1 Envelope Glycoproteins lacking Palmitoylated Cysteines in the gp41 Cytoplasmic Domain: Influence on Cell Surface Expression, Incorporation onto Virions and Infectivity
J Bhattacharya*, P Peters, and P Clapham
Univ. of Massachusetts Med. Sch., Worcester, USA
294C-terminal Truncations of the HIV-1 gp41 Define a Minimal Membrane Anchor and Toplogical Model for the Membrane-Spanning Domain
L Yue, N Brown, and E Hunter*
Univ. of Alabama at Birmingham, USA
295Formation of a Salt Bridge between the N- and C-terminal Heptad Repeats of HIV-1 gp41 Is Critical for Stabilization of 6-Helix Bundle and Virus Fusion
Y He*, S Liu, A K Debnath, and S Jiang
Lindsley F. Kimball Res. Inst., New York Blood Ctr., NY, USA
296The Membrane Proximal Tyrosine-Based Sorting Signal of HIV-1 gp41 Is Required for Optimal Viral Infectivity and Viral Entry
J Day*1, and J Guatelli1,2
1Univ. of California, San Diego, USA and 2San Diego VA Healthcare System
297Dually Inducible Cell Lines to Study the Quantitative CD4 and Co-receptor Dependence of HIV Envelopes
S Baik, and B Lee*
Univ. of California, Los Angeles-MIMG, USA
298The HIV-1 Envelope Proteins of Patients Isolates with Naturally Occurring Cytoplasmic Tail Deletions Exhibit Altered Membrane Fusion and Infectivity
W Huang*, J Toma, J Whitcomb, S Fransen, T Wrin, E Penuel, and C Petropoulos
ViroLogic Inc., South San Francisco, CA, USA
299HIV Envelope-induced Syncytium Formation Is Dependent on Actin Cytoskeletal Reorganization and Co-receptor-mediated Activation of Rac-1 GTPase
S Pontow*, N Vander Heyden, and L Ratner
Washington Univ. Sch. of Med., St. Louis, MO, USA
300Env Content in Virions as an Important Determinant of Relative Sensitivity to Antibody-mediated Neutralization
E Yuste*, and R Desrosiers
New England Primate Res. Ctr., Harvard Med. Sch., Southborough, MA, USA
301Use of Fc-gp120 Adhesins To Characterize Receptor Interactions
A de Parseval*, J Binley, S Ngo, D Burton, and J Elder
Scripps Res. Inst., La Jolla, CA, USA
302
Semen-specific Genetic Characteristics of HIV-1 env
S Pillai*1, B Good1, J Wong1, M Strain1, D Richman1,2, and D Smith1
1Univ. of California, San Diego, USA and 2VA San Diego Healthcare System, La Jolla, CA, USA
303
Identification of High-mass Oligomers of DC-SIGN on Monocyte-Derived Dendritic Cells and Preferential Recognition by Mannan and HIV-gp120
O K Bernhard*1, J Wilkinson1, M M Sheil2, and A L Cunningham1
1Ctr. for Virus Res., Westmead, Australia and 2Univ. of Wollongong, Australia
304Identification of Monoclonal Antibodies that Define HIV-1 Immunotypes
A Nadas1, P Zhong1, S Burda1, L Zekeng2, M Gorny1, and P Nyambi*1
1New York Univ. Sch. of Med., NY, USA and 2Lab. de Sante Hygiene Mobile, Yaounde, Cameroon
305Inhibition of HIV-1 Entry: Broadly HIV-1 Neutralizing Human Monoclonal Antibodies and Their Derivatives
M-Y Zhang*, and D S Dimitrov
HIVCB, LECB, NCI-Frederick, NIH, DHHS, Frederick, MD, USA
306Rapid Screening of Subtypes B and C Neutralizing Antibody Responses Against PBMC Grown HIV-1 Isolates in an Engineered CEM Cell Line
Y Sun*1, V Sharma1, I Srivastava1, E Kan1, N Landau2, and S Barnett1
1Chiron Corp., Emeryville, CA, USA and 2Salk Inst., San Diego, CA, USA
307HIV-1 Envelope Determinants of CXCR4-mediated Infection of Macrophages: Utilization of CXCR4 by HIV-1 Is Cell-type-specific
D Tuttle1, G Ghaffari*1, L Jeffers1, D Briggs1, J Sleasman2, and M Goodenow1
1Univ. of Florida, Gainesville, USA and 2Univ. of South Florida and All Children's Hosp., St. Petersburg, USA