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Session 65   Poster Abstracts
Viral Targets and Reservoirs
Tuesday, 1:30 - 3:30 pm
Poster Hall
416Characterization of DC-SIGN and DC-SIGNR Transcript Isoforms in Human Vaginal and Rectal Mucosa
H Liu*1, F Hladik2, T Andrus1, P Nelson2, P Sakchalathorn2, G Lentz1, L Corey1,2, M J McElrath1,2, and T Zhu1
1Univ. of Washington Sch. of Med., Seattle, USA and 2Fred Hutchinson Cancer Res. Ctr., Seattle, WA, USA
417Lack of SIV Target Cells in African Non-human Primate: Host or Virus Adaptation?
I Pandrea*, C Apetrei, J Dufour, M Mefford, R Bohm, P Marx, A Lackner, and R Veazey
Tulane Natl. Primate Res. Ctr., Covington, LA, USA
418Differences in Dendritic Cell Populations and Signaling May Account for Divergent SIV Infection Outcomes in Rhesus Macaques and Sooty Mangabeys
A P Barry*, R Chavan, M Wernett, M McCausland, S Staprans, G Silvestri, and M B Feinberg
Emory Univ., Atlanta, GA, USA
419Parallel Rather Than Sequential Targeting of Intraepithelial Langerhans and T Cells during Vaginal HIV-1 Transmission
F Hladik*1, P Sakchalathorn1, L Ballweber1, G Lentz2, D Eschenbach2, and M J McElrath1
1Fred Hutchinson Cancer Res. Ctr., Seattle, WA, USA and 2Univ. of Washington, Seattle, USA
420
The Distal Lower Genitourinary Tract as a Source of Seminal HIV
R Coombs*, L Deutsch, S Ross, J Dragavon, S Harb, A Motoshige, L Stensland, K Diem, M Hooton, A Collier, and J Krieger
Univ. of Washington, Seattle, USA
421HIV-1 Cervicovaginal Reservoirs in the Era of HAART
G Nunnari*1, J Kulkosky1, J Sullivan1, Y Xu1, W Cavert2, P Nyirjesy1, I Frank3, and R J Pomerantz1
1Ctr. for Human Virology and Biodefense, Thomas Jefferson Univ., Philadelphia, PA, USA; 2Univ. of Minnesota, Minneapolis, USA; and 3Univ. of Pennsylvania, Philadelphia, USA
422The Presence of Extracellular Virion-associated HIV-RNA in Lymphoid Tissue Reflects Local Productive Infection: A Detailed Analysis of Lymphatic HIV Transcription Patterns in vivo
M Fischer*1, B Joos1, J K Wong2, B Hirschel3, M Opravil1, R Weber1, H F Guenthard1, and The Swiss HIV Cohort Study
1Univ. Hosp. Zurich, Switzerland; 2Univ. of California, San Diego and VA Med. Ctr., La Jolla, CA, USA; and 3Univ. Hosp. Geneva, Switzerland
423Expression of Latent HAART-persistent HIV-1 Induced by Novel Cellular Activating Agents: Molecularly Interrogating HIV-1 Reservoirs
R Pomerantz*1, J Sullivan1, Y Xu1, E Souder1, D Hamer2, and J Kulkosky1
1Thomas Jefferson Univ., Philadelphia, PA, USA and 2NCI, NIH, DHHS, Bethesda, MD, USA
424Purging HIV-1 Viral Reservoirs: The Role of IL-7
F X Wang*1, Y Xu1, J Sullivan 1, J Fisher1, E Souder1, E Acheampong1, E Argyris1, I Frank2, J Kulkosky1, R J Pomerantz1, and G Nunnari1
1Ctr. for Human Virology and Biodefense, Thomas Jefferson Univ., Philadelphia, PA, USA and 2Univ. of Pennsylvania, Philadelphia, USA
425Actinomycin D and IL-6 Synergistically Stimulate HIV-1 Replication in a Latently Infected Cell Line U1 through Activating MRP8
Y Liu*1, R A Lempicki1, H C Lane2, and T Imamichi1
1SAIC-Frederick, Inc., MD, USA and 2NIAID, NIH, DHHS, Bethesda, MD, USA
426
CD14lo/CD16hi Monocytes are More Permissive to HIV-1 Infection in vitro and May Selectively Harbour HIV-1 in Patients on HAART
P Ellery1,2, C Sonza1, G Paukovics1, P U Cameron3, A Solomom2,4, SR Lewin2,4, S M Crowe*1,2,4,5, and
1Macfarlane Burnet Inst. for Med. Res. and Publ. Hlth., Melbourne, Australia; 2Monash Univ., Melbourne, Australia; 3Univ. of Melbourne, Australia; 4Alfred Hosp., Melbourne, Australia; and 5Natl. Ctr. for HIV Virology Res., Melbourne, Australia
427
Quantitation of Rare HIV-1 Integration Events in PBMC from HIV-1-infected Individuals Using Repetitive Sampling Techniques
J Yu1, W J Swiggard1, T Theodosopoulos2, and U O'Doherty*1
1Univ. of Pennsylvania, Philadelphia, USA and 2Drexel Univ., Philadelphia, PA, USA
427bAn Anti-CD45RO Immunotoxin Kills Latently Infected, Replication-competent, CD4+ T Cells Purified from HIV+ Individuals on HAART with Viremia below the Limit of Detection
J Saavedra-Lozano*1, Y Cao1, J Callison1, R Sarodi1, D Peterson1, L Picker2, O Ramilo1, and E Vitetta1
1Univ. of Texas Southwestern Med. Ctr., Dallas, USA and 2Oregon Hlth. and Sci. Univ., Portland, USA
427cCoaxing HIV-1 from Resting CD4+ T Cells: Valproic Acid Induces Latent Viral Expression
D M Margolis*1,2, G Lehrman1, N M Archin1, L Ylisastigui1, M B Kvanli2, D Turner2, J Wagner2, H Wise2, and R J Bosch3
1Univ. of Texas Southwestern Med. Ctr., Dallas, USA; 2North Texas Veterans Hlth. Care Systems, Dallas, USA; and 3Harvard Sch. of Publ. Hlth., Boston, MA, USA